√99以上 a-431 cells 211482-A431 cells

 Cell surface EGFR of 31 cells were labeled on ice with either EGFR or TfnRspecific monoclonal antibody, and internalized for 4 h either in the absence or presence of aspirin Cells were washed with cold PBS to remove aspirin, incubated at various timepoints with fresh media without aspirin and detected for the internalized endocytosedEGFR and endocytosed31 A 431 Description Applications This cell line is neither produced nor fully characterized by ATCC We do not guarantee that it will maintain a specific morphology purity or any other property upon passage Catalog Number CRL7907 Price Epidermal growth factor (EGF) rapidly stimulates receptor autophosphorylation in 31 cells After 1 min the phosphorylated receptor can be identified at the plasma membrane using an anti phosphotyrosine antibody With further incubation at 37 degrees C, approximately 50% of the phosphorylated EGF

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A431 cells-Staining of U 2OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cellCell Tissue Res (03) –410 DOI /s REGULAR ARTICLE Natalia A Gloushankova Tetsuro Wakatsuki Regina B Troyanovsky Elliot Elson Sergey M Troyanovsky Continual assembly of desmosomes within stable intercellular contacts of epithelial 31 cells Received / Accepted 5 September 03 / Published online 16 October 03

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31 (The above parameters are from one study For further information on this cell line and other parameters, including different strains, vendors, implant type and location and/or standards of care, please contact us) ***Please note that cell lines areEpidermoid carcinoma) Human 31 cells were cultured in DMEM medium and harvested at the log phase of growth The 31 cells were fixed in 4% paraformaldehyde and arrayed on a 12well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining31 cells were seeded in 6well plates and treated for 48 h with curcumin (5, 10, 25, 50 and 100 μM) Representative pictures of untreated and treated 31 cells Phase contrast and fluorescent (Hoechst ) pictures of the control and treated samples Scale bar 100 μM To evaluate cell viability, the epidermoid carcinoma cell line, A

31 cells and the clonal variants were each cultured in soft agar (10,000 cells/16mm well), as described in "Materials and Methods" The cultures con tained DME supplemented with 10% FBS with and without EGF (30 ng/ml) as indicated Colony numbers were determined on day 8J Cell Biol ) Here, the purification of ezrin from human placenta is described Antibodies to human ezrin, together with antibodies to other microfilamentassociated proteins, were used to follow the distribution and phosphorylation of these proteins in 31 cellsWhile 31 cells constitutively express ICAM1 when assessed by northern blotting, by biosynthetic labeling and immunoprecipitation, and by flow cytometry, HK do not When these two cell types are exposed to recombinant human (rh) IFNgamma at 1000 U/ml for 24 h,

31 Cells 31 cells are derived from an 85year old female epidermis The epidermoid carcinoma cell line expresses very high levels of the epidermal growth factor (EGF) receptor, and displays a high basal activation of the MAPK pathwayTransfection and selection data of 31 cells by various antibiotics in differing mediaCell No JCRB0004 Cell Name 31 LOT No Lot Specification distribution Medium Dulbecco's modified Eagle's medium (high glucose) with 10% fetal bovine serum (FBS;

Peroxynitrite Induces Covalent Dimerization Of Epidermal Growth Factor Receptors In 31 Epidermoid Carcinoma Cells Journal Of Biological Chemistry

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Western Blot 31 Whole Cell Lysate NB800PC12 Coommassie stain of 002 ml of NB 800PC12 separated in a 4% gradient gel under nonreducing conditions (lane 1) MW standard isAddition of EGF to human carcinoma 31 cells is known to induce membrane ruffling after approximately 2 min (Chinkers, M, J A McKanna, and S Cohen 1979 J Cell Biol ) and the phosphorylation of a protein referred to as p81, a known substrate for various proteintyrosine kinases (Cooper, J A, D F BowenPope, E Raines, R Ross, and T Hunter 19There are over thirty cancer cell lines that are used in epidermoid and squamous cell carcinoma research including 31 The 31 cell line was established from the epidermis (skin) of an 85yearold female patient who suffered from epidermoid carcinoma These cells exhibit an epithelial morphology and are tumorigenic

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 The basal level of intracellular cyclic AMP (cAMPi) in 31 cells incubated at 37 degrees C in Na()containing Hanks solution is 86 / 139 fmol/10(6) cellsThis cell line was established through single cell cloning, and the cells constitutively express high levels of enzymatically active luciferase protein, which can be detected via in vitro and in vivo bioluminescence assays The cells should be maintained in Blasticidin (8 µg/mL) containing medium in routine cell culture /jcpabs The metabolism of the receptor for epidermal growth factor (EGF) in A‐431 cells has been measured by labeling the receptor in vivo with radioactive amino acid precursors and then determining, by immunoprecipitation with specific anti‐EGF receptor antisera, the rate of degradation of the receptor when the cells are placed in a

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Detailed information about the celline expression of DPY19L1 on RNA level in 31 31 cells were chosen as it is a cancer cell line, and expresses our receptors of interest, EGFR (Wrann and Fox, 1979) and TfR (Hopkins and Trowbridge, 19), at high levels In this study, we showed that andrographolide causes the downregulation of EGFR from the cell surface as well as the inhibition of EGFR and TfR degradation9 rows 31 cells are grown on DMEM containing 10% (v/v) FBS until near confluency and then incubated in

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 title = "Increases in HSF1 translocation and synthesis in human epidermoid 31 Cells role of protein kinase C and Ca2i", abstract = "Background It is known that heat shock increases both heat shock protein 70 kd (HSP70), mRNA synthesis, and intracellular cytosolic free calcium concentration ( Ca2i)Cell Any 31 A549 AN3CA BEWO CACO2 CAPAN2 Daudi EFO21 HaCaT HDLM2 HEK 293 HEL HeLa Hep G2 HL60 HMC1 HTh K562 Karpas707 Leukemia, AML Leukemia, BALL Leukemia, BCLL Leukemia, CML Leukemia, TALL LP1 MCF7 MOLT4 NB4 NTER PBMC PC3 REH RH30 RPMI26 RT4 SCLC21H SHSY5Y SiHa SKBR3 SKMEL30 THP1 TIME U138Flurbiprofen benzyl nitrate (NBS242) inhibits the growth of 31 human epidermoid carcinoma cells and targets ßcatenin Niharika Nath Related Papers The proapoptotic effect of traditional and novel nonsteroidal antiinflammatory drugs in mammalian and yeast cells

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Cell atlas Showing subcellular location of SRRM2 (Cwc21, KIAA0324, SRL300, SRm300) The 31 cell line, which was derived from an epidermoid carcinoma excised from the skin tissue of an 85yearold female, is one of a succession of cell lines established from solid tumors by a research team led by D J Giard The epithelial line is tumorigenic, forming rapidly growing subcutaneous tumors in immunosuppressed mice and colonies in31 cells by Hunter and Cooper (1981) and found to be phosphorylated 2 min after addition of EGF, although its function and location was unknown Over the last 10 yr we have been studying the molecular structure of the microvillar cytoskeleton of chicken intestinal epithelial cells (Bretscher

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 31 may refer to 31 road England a road between Bath and Bristol 31 cells an experimental cell line used in biomedical research Kurloff cells also known as Foà Kurloff cells are cells found in the blood and organs of guinea pigs and capybara, contain large secretory granules transcripts encoding platelet derived endothelial cell growth factor thymidine phosphorylase in 31 cells We established two erlotinibresistant pools of 31 cells, a wellcharacterized epidermoid cancer cell line that constitutively overexpresses EGFR and is sensitive to erlotinib, by continuous exposure to erlotinib over a 6month periodDownload scientific diagram Ceramide induced cell death 31 cells cultured in medium containing 10% FCS were treated with C 8 ceramide (25 m M) for from publication Ceramide Induces Caspase

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ATCC a431 cells 31 Cells, supplied by ATCC, used in various techniques Bioz Stars score 99/100, based on 712 PubMed citations ZERO BIAS scores, article reviews, protocol conditions andThe epidermoid carcinoma cell line 31 expresses abnormally high levels of epidermal growth factor receptor (EGFR) and is a useful model for studying EGFRmediated signaling, which regulates growth, survival, proliferation, and differentiation in mammalian cells Abstract 31 human epidermoid carcinoma cells in culture may be grown in the absence of serum in a one to one mixture of Ham's F12 and Dulbeccomodified Eagle's medium supplemented with 10 μg/ml bovine insulin, 10 μg/ml human transferrin, 5 μg/ml human coldinsoluble globulin and 05 mM ethanolamine

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Horseradish peroxidase (HRP) uptake was used to measure fluidphase pinocytosis in monolayers of human epithelioid carcinoma cells (31) Histochemistry confirmed that cellassociated HRP was restricted to intracellular vesicles Biochemical methods showed that HRP uptake in control cultures was directly proportional to the duration of exposure31 Cells and Human Keratinocytes Synthesize and Secrete the Third Component of Complement Nicole BassetSCguin, S Wright Caughman, and Kim B Yancey Department of Dermatology (NBS, KBY), Uniformed Services University of theSigma Cat # 1712) Temperature 37 C Cell Density at Seeding 10 12 x 10^5 cells/mL Methods for Passages Cells were harvested after treatment with 025

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Cell atlas Showing subcellular location of KRT13 (CK13, K13, MGC, MGC3781)5 rows Every step of the way, a wide range of cell health products Maintaining healthy cells is the keyRecommended control Human wildtype 31 cell line ( ab ) Please note a wildtype cell line is not automatically included with a knockout cell line order, if required please add recommended wildtype cell line at no additional cost using the code WILDTYPETMTK1 Cryopreservation cell medium Cell Freezing MediumDMSO Serum free media,

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31 cells were cultured under the indicated conditions, and were lyzed in the buffer containing mM Tris–HCl (pH 74), 1 mM ethylenediamine tetraacetic acid, 1 µg aprotinin per ml, 1 µg pepstatin per ml, 1 µg leupeptin per ml, 15 mM benzamidine, and 375 mM βmercaptoethanolVascularendothelial cell growthfactor (VEGF)producedby31 humanepidermoidcarcinomacells andidentification ofVEGF membranebindingsites* (angiogenesis/tumor cells) YOSHINARI MYOKEN,YOSHIAKI KAYADAt, TETSUJI OKAMOTOt,MIKIo31 Cell Lines Your search returned 3 31 Cell Lines Cells and Microorganisms across 2 suppliers Select up to 5 products from below to compare or request more information

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Lane 1 Wildtype 31 (Human epidermoid carcinoma cell line) whole cell lysate Lane 2 HLAA knockout 31 (Human epidermoid carcinoma cell line) whole cell lysate Lane 3 Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate Performed under reducing conditions Lanes 1 3 Merged signal (red and green) However, in contrast to other cell types in which the receptorbound LDL is internalized with high efficiency, in the 31 cells only a small fraction of the receptorbound LDL entered the cell In CHO cells approximately 66% of the LDL receptors were located over coated regions of membrane, and the efficiency of LDL internalization was correspondingly 10fold higher than in 31 cells

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